Southern: detecting specific DNA sequences after gel electrophoresis

What changed

Transferred size-separated DNA fragments to a filter and probed complementary sequences by labeled hybridization.

Scientific scope

The record is Southern's sole-author 1975 Journal of Molecular Biology paper. It covers the reported DNA-transfer and hybridization method; restriction-enzyme discovery, later blot variants, nonradioactive detection and clinical assays remain separate.

Evidence

The linked sources identify this contribution and its scope. WRS has not independently reconstructed the scientific claims. The record is Southern's sole-author 1975 Journal of Molecular Biology paper. It covers the reported DNA-transfer and hybridization method; restriction-enzyme discovery, later blot variants, nonradioactive detection and clinical assays remain separate.

Contributors

• E. M. Southern · SCIENCE: Named source author of the scoped work; individual effort shares are not measured.

• PubMed record for Southern DNA-transfer paper
• ScienceDirect page for Southern DNA-transfer paper

Provisional WRS assessment, edition 2026-10-16.1. Independent expert calibration is pending. This grade does not certify correctness or assign ownership.

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