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Southern: detecting specific DNA sequences after gel electrophoresis

Described transferring electrophoretically separated DNA fragments from agarose gels to nitrocellulose filters and detecting complementary sequences by labeled hybridization.

What is recorded here?

Described transferring electrophoretically separated DNA fragments from agarose gels to nitrocellulose filters and detecting complementary sequences by labeled hybridization.

Source-linked catalogue note. A detailed explanatory guide has not yet been prepared for this entry.

Scientific scope

The record is Southern's sole-author 1975 Journal of Molecular Biology paper. It covers the reported DNA-transfer and hybridization method; restriction-enzyme discovery, later blot variants, nonradioactive detection and clinical assays remain separate.

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People and systems

E. M. Southern

The named authors contributed to this scoped work; individual effort shares and later applications are not assigned by the byline.

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Documented countries / regions: United Kingdom

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English catalogue narrative

Southern: detecting specific DNA sequences after gel electrophoresis

Described transferring electrophoretically separated DNA fragments from agarose gels to nitrocellulose filters and detecting complementary sequences by labeled hybridization.

Scientific scope

The record is Southern's sole-author 1975 Journal of Molecular Biology paper. It covers the reported DNA-transfer and hybridization method; restriction-enzyme discovery, later blot variants, nonradioactive detection and clinical assays remain separate.

Reading status

This is a source-linked WRS catalogue note. A full explanatory guide and independent scientific reconstruction have not been completed for this entry. Read the identified original work for its complete narrative and arguments.

People and systems

E. M. Southern

The named authors contributed to this scoped work; individual effort shares and later applications are not assigned by the byline.

Original sources