CRISPR–Cas9: a programmable RNA-guided nuclease

What changed

RNA sequence could direct programmable DNA cleavage in the demonstrated system.

Scientific scope

The 2012 biochemical Cas9 mechanism and engineered single-guide RNA described by Jinek and colleagues.

Evidence

The cited historical record or published author account supports this scoped contribution. The source-reading boundary is stated at each link; a WRS grade is not an independent scientific validation.

Contributors

• Jinek, Chylinski, Fonfara, Hauer, Doudna and Charpentier · SCIENCE: The recorded biochemical mechanism paper; earlier and later groups remain distinct.

• Jinek et al., 2012

Provisional WRS assessment, edition 2026-10-16.1. Independent expert calibration is pending. This grade does not certify correctness or assign ownership.

Full scientific record · Browse contribution grades · Request a correction