What is recorded here?
Established long-term culture conditions in which single Lgr5-positive intestinal stem cells generated self-organizing crypt-villus organoids containing differentiated epithelial cell types without a mesenchymal niche.
Source-linked catalogue note. A detailed explanatory guide has not yet been prepared for this entry.
Scientific scope
The record is the exact 2009 Nature paper using mouse intestinal cells. Earlier three-dimensional cultures, later human organoids, disease models, transplantation, drug testing and clinical efficacy claims remain separate.
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People and systems
Toshiro Sato; Robert G. Vries; Hugo J. Snippert; Marc van de Wetering; Nick Barker; Daniel E. Stange; Johan H. van Es; Arie Abo; Pekka Kujala; Peter J. Peters; Hans Clevers
The named authors contributed to this scoped work; individual effort shares and later applications are not assigned by the byline.
Research locations and affiliations
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Documented countries / regions: Netherlands, United States
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English catalogue narrative
Sato and colleagues: single Lgr5 stem cells form crypt-villus organoids
Established long-term culture conditions in which single Lgr5-positive intestinal stem cells generated self-organizing crypt-villus organoids containing differentiated epithelial cell types without a mesenchymal niche.
Scientific scope
The record is the exact 2009 Nature paper using mouse intestinal cells. Earlier three-dimensional cultures, later human organoids, disease models, transplantation, drug testing and clinical efficacy claims remain separate.
Reading status
This is a source-linked WRS catalogue note. A full explanatory guide and independent scientific reconstruction have not been completed for this entry. Read the identified original work for its complete narrative and arguments.
People and systems
Toshiro Sato; Robert G. Vries; Hugo J. Snippert; Marc van de Wetering; Nick Barker; Daniel E. Stange; Johan H. van Es; Arie Abo; Pekka Kujala; Peter J. Peters; Hans Clevers
The named authors contributed to this scoped work; individual effort shares and later applications are not assigned by the byline.