BWhat changed
An existing programmable nuclease became more selective in the reported experiments.
Scientific scope
The structure-guided eSpCas9 variants and their specificity/on-target tradeoff assessed in the Slaymaker et al. 2015-online / 2016-journal study.
Evidence
The author manuscript’s byline, main results and Figures 2–3 were checked. It reports targeted and genome-wide off-target analyses. WRS did not rerun the experiments or audit all supplements.
Contributors
- Ian M. Slaymaker and Linyi Gao · SCIENCE: Named equal-contribution authors of the paper.
- Bernd Zetsche, David A. Scott, Winston X. Yan and Feng Zhang · SCIENCE: Other named authors; no numerical division of their contributions is inferred.
- Slaymaker et al., Rationally engineered Cas9 nucleases with improved specificity
- Original publication metadata
Provisional WRS assessment, edition 2026-10-28.1. Independent expert calibration is pending. This grade does not certify correctness or assign ownership.